Clavibacter michiganensis subsp.|BNCC337450 |BNCC

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Clavibacter michiganensis subsp.

Culture medium	Tryptone soybean blood plate (TSA-B): tryptone 15.0g, soybean peptone 5.0g, sodium chloride 5.0g, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate evenly. (5) put the plates under the growth conditions.
Growth conditions	30 ℃;24-48h; Aerobic
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: Yellow Protuberance: Middle convex Surface: Bright and Smooth Texture: Wet and Easy to Stir
Sharing mode	Public welfare sharing

Clavibacter michiganensis subsp. michiganensisi

Storage conditions : 2~8 ℃

No. : 337450

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :30 ℃, aerobic, blood plate medium (TSA + 5% defibrinogen sheep blood),24-48h.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 24-48hours.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 48h strain layer become obvious, colony is typical
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque color: yellow uplift: middle convex surface: bright and smooth texture: moist and easy to stir
Conclusion:	good viability, no abnormal colony morphology, qualified